ABSTRACT
Objective:To examine the relationship between brain natriuretic peptide (BNP) and the recurrence of atrial fibrillation (AF) after successful electrical cardioversion (EC) by a meta analysis approach.Methods:Based on PubMed,Medline and China National Knowledge Infrastructure (CNKI) databases,related literatures,published from 1980-2008,were searched; a manual search was also performed.The included trials were analyzed by RevMan4.2 software for meta analysis.Results:Totally seven studies were finally included.The analysis revealed that the baseline BNP level were greater in patients with AF recurrence than those without AF recurrence,with the standardized mean difference in the BNP levels being 0.82 units (95% confidence interval 0.12 to 1.52).The test for overall effect Z-score was 2.30,with a P value of 0.02; there was significant difference.Conclusion:It is suggested that the higher BNP level after successful electrical cardioversion is associated with greater risk of AF recurrence.The level of BNP may serve as an indicator for AF recurrence after successful EC.
ABSTRACT
Objective:To prepare monoclonal antibody against Annexin B for further studying the physiological function of Annexin B1.Methods: Annexin B1 was expressed and purified in E.coli and its antigenicity was confirmed.The product was used to immunize BALB/c mice by intrasplenic embedding.Spleen cells of BALB/c mice were obtained and fused with myeloma cell line SP2/0 in a HAT medium containing 50% PEG 4000 and were then subjected to incubation in 37℃,5% CO_(2)incubator for several days.ELISA was used to assay the type and titer of the McAbs in the supernatant.Results: A hybridoma cell line 2B10H5,which can steadily secrete specific McAbs,was obtained.Conclusion: The McAbs of Annexin B1 have been successfully prepared,which pave a way for further investigation of Annexin B1.